Heparin Binding Protein EIA
The Axis-Shield Heparin Binding Protein (HBP) assay is an enzyme immunoassay (EIA) for the quantitative determination of Heparin Binding Protein in human plasma.
The microtitre break-apart wells are coated with a monoclonal antibody to Heparin Binding Protein (HBP). During the first incubation, HBP in the sample specifically binds to the antibody-coated surface. The wells are then washed to remove unbound components. In the second incubation the Conjugate binds to any captured HBP. After further washing the bound HBP is detected by incubation with the Substrate. Addition of the Stop Solution terminates the reaction, resulting in a coloured end-product. The concentration of HBP in ng/mL is directly related to the colour generated and can be estimated by interpolation from a dose-response curve based on Calibrators.
The determination of HBP is based on a turbidimetric reaction which occurs between circulating Heparin Binding Protein and avian HBP monospecific polyclonal antibodies bound to chloromethyl microparticles at optimal pH conditions in the presence of polyethylene glycol polymer (PEG). The magnitude of the change is proportional to the quantity of HBP in the test sample.
